Simple Preparation of Green Fluorescent Protein Conjugated with β-Cyclodextrin in a Site Specific Manner

Miho SUZUKI; Yoshihiro ISHIMARU; Ayumu SAITO; Koichi NISHIGAKI

doi:10.2116/analsci.29.811

Original Papers

Simple Preparation of Green Fluorescent Protein Conjugated with β-Cyclodextrin in a Site Specific Manner

Miho SUZUKI, Yoshihiro ISHIMARU, Ayumu SAITO, Koichi NISHIGAKI

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Keywords: Fluorescent protein, β-cyclodextrin, supramolecule, drug delivery, fluorescence resonance energy transfer (FRET)

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Supplementary material

2013 Volume 29 Issue 8 Pages 811-814

DOI https://doi.org/10.2116/analsci.29.811

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Abstract

We have site-directedly linked a green fluorescent protein (GFP) variant and a β-cyclodextrin (β-CD) with a simple method to develop a basic complex for sophisticated supramolecules. We have confirmed β-CD grafting on GFP with several methods including matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry (MALDI-TOF MS) without protease digestion and characterized the complex as well. In consideration of the resulting properties, the product we plainly and efficiently obtained could have applications related to sensing devices and drug delivery systems.

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