Differences in the Substrate Specificities and Active-Site Structures of Two α-<small>L</small>-Fucosidases (Glycoside Hydrolase Family 29) from Bacteroides thetaiotaomicron

Haruko SAKURAMA; Erika TSUTSUMI; Hisashi ASHIDA; Takane KATAYAMA; Kenji YAMAMOTO; Hidehiko KUMAGAI

doi:10.1271/bbb.111004

Biochemistry & Molecular Biology Notes

Differences in the Substrate Specificities and Active-Site Structures of Two α-L-Fucosidases (Glycoside Hydrolase Family 29) from Bacteroides thetaiotaomicron

Haruko SAKURAMA, Erika TSUTSUMI, Hisashi ASHIDA, Takane KATAYAMA, Kenji YAMAMOTO, Hidehiko KUMAGAI

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Keywords: 1,3-1,4-α-L-fucosidase (EC 3.2.1.111), α-L-fucosidase (EC 3.2.1.51), glycoside hydrolase family 29

JOURNAL FREE ACCESS

2012 Volume 76 Issue 5 Pages 1022-1024

DOI https://doi.org/10.1271/bbb.111004

View "Advance Publication" version (May 7, 2012).

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Abstract

Recent studies suggest that α-L-fucosidases of glycoside hydrolase family 29 can be divided into two subfamilies based on substrate specificity and phylogenetic clustering. To explore the validity of this classification, we enzymatically characterized two structure-solved α-L-fucosidases representing the respective subfamilies. Differences in substrate specificities are discussed in relation to differences in active-site structures between the two enzymes.

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