Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Regular Paper
The Involvement of Mitogen-Activated Protein Kinases in the 1α,25-Dihydroxy-Cholecalciferol-Induced Inhibition of Adipocyte Differentiation In Vitro
Satoru SAKUMAJunji FUJISAWAMaki SUMIDAMami TANIGAWARie INODATakuya SUJIHERATetsuya KOHDAYohko FUJIMOTO
Author information
JOURNAL FREE ACCESS

2012 Volume 58 Issue 1 Pages 1-8

Details
Abstract

The present study was undertaken to investigate the mechanism by which 1α, 25-dihydroxy-cholecalciferol [1α,25-(OH)2-VD3] modulates the differentiation of mouse 3T3-L1 preadipocytes into mature adipocytes. Treatment with 1α,25-(OH)2-VD3 in the presence of insulin, dexamethasone and 3-isobutyl-1-methyl-xanthine significantly inhibited the triacylglycerol accumulation, and mRNA expressions of adipocytokines (adiponectin and tumor necrosis factor-α) and plasminogen activator inhibitor-1 in the pico-nanomolar concentration range, indicating that 1α,25-(OH)2-VD3 under physiological conditions inhibits the differentiation of 3T3-L1 cells. 1α,25-(OH)2-VD3 potently reduced the mRNA and/or protein expressions of CCAAT-enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ), and the nuclear translocation of PPARγ. Furthermore, it inhibited the mRNA expression and phosphorylation of extracellular signal-regulated kinase (ERK), one of mitogen-activated protein kinases. These results indicate that 1α,25-(OH)2-VD3 can be an inhibitor of adipocyte differentiation, and suggest, in addition to C/EBPα and PPARγ, an important role of ERK in mediating 1α,25-(OH)2-VD3-induced alteration in adipocyte differentiation.

Content from these authors
© 2012 by the Center for Academic Publications Japan
Next article
feedback
Top