Scanning ion conductance microscopy (SICM), first introduced by Hansma et al. (1989), can obtain topographic images of the sample surface and has been expected to be useful for biological studies. In this paper, we explained the principle of the SICM and showed some results of the application of SICM to biology. We showed SICM images of collagen fibrils, chromosomes and cultivated cells in liquid. We then applied SICM for imaging the surface of tissue blocks (e.g., trachea, etc.). Sequential time-lapsed SICM images of live cells were also shown for revealing the movement of cellular processes on the time scale of minutes. The advantages of SICM imaging in biology were discussed by comparison with atomic force microscopy (AFM) and/or scanning electron microscopy (SEM) images.