Nanostructures with chiral shapes show optical activity. The optical activity of the chiral nanostructure may be regarded approximately as an integration of contributions from optical activity of local sites over the entire nanostructure. In this article, we describe our recent experimental efforts to realize 100-nm-scale spatially resolved circular dichroism (CD) imaging. The results of CD imaging for S-shaped gold nanostructure are compared with the macroscopically obtained CD spectra. Local CD signals of both handednesses coexisted in the individual nanostructure, and the spatial distribution of the CD reflected the chiral symmetry of the nanostructure. When the local CD signal is integrated over the entire nanostructure, its value was approximately 1% of the maximum local CD signal, which roughly coincided with the macroscopic CD signal. This indicates that there are possibly prominent local CD signals even if only a tiny CD signal is observed in the macroscopic measurements of optical activity for nanostructured samples.