Histopathological Evaluation of the Efficacy of Antifungals for Experimental Trichosporon Bloodstream Infection

Daisuke Sasai; Yoichiro Okubo; Takao Ishiwatari; Takashi Sugita; Takehiko Kaneko; Somay Yamagata Murayama; Tsuyoshi Shimamura; Minoru Shinozaki; Chikako Hasegawa; Aki Mitsuda; Naobumi Tochigi; Megumi Wakayama; Tetsuo Nemoto; Kazutoshi Shibuya

doi:10.7883/yoken.66.133

Original Article

Histopathological Evaluation of the Efficacy of Antifungals for Experimental Trichosporon Bloodstream Infection

Daisuke Sasai, Yoichiro Okubo, Takao Ishiwatari, Takashi Sugita, Takehiko Kaneko, Somay Yamagata Murayama, Tsuyoshi Shimamura, Minoru Shinozaki, Chikako Hasegawa, Aki Mitsuda, Naobumi Tochigi, Megumi Wakayama, Tetsuo Nemoto, Kazutoshi Shibuya

Author information

Daisuke Sasai
Department of Surgical Pathology, Toho University Omori Medical Center
Yoichiro Okubo
Department of Surgical Pathology, Toho University Omori Medical Center
Takao Ishiwatari
Department of Surgical Pathology, Toho University Omori Medical Center
Takashi Sugita
Department of Microbiology, Meiji Pharmaceutical University
Takehiko Kaneko
Laboratory of Human Nutrition, Wayo Women's University
Somay Yamagata Murayama
Laboratory of Molecular Cell Biology, School of Pharmacy, Nihon University
Tsuyoshi Shimamura
Department of Surgical Pathology, Toho University Omori Medical Center
R&D Laboratories, Pola Pharma Inc.
Minoru Shinozaki
Department of Surgical Pathology, Toho University Omori Medical Center
Chikako Hasegawa
Department of Surgical Pathology, Toho University Omori Medical Center
Aki Mitsuda
Department of Surgical Pathology, Toho University Omori Medical Center
Naobumi Tochigi
Department of Surgical Pathology, Toho University Omori Medical Center
Megumi Wakayama
Department of Surgical Pathology, Toho University Omori Medical Center
Tetsuo Nemoto
Department of Surgical Pathology, Toho University Omori Medical Center
Kazutoshi Shibuya
Department of Surgical Pathology, Toho University Omori Medical Center

JOURNAL FREE ACCESS

2013 Volume 66 Issue 2 Pages 133-139

DOI https://doi.org/10.7883/yoken.66.133

Details

Abstract

The efficacy of polyene macrolides to treat experimental Trichosporon bloodstream infection was evaluated by histopathological examination and viable cell counts in the kidneys of infected mice. Viable cell counts on the 5th day after infection confirmed that liposomal amphotericin B (L-AMB) is a more effective treatment than fluconazole (FLC) for mice infected with an azole-resistant strain of Trichosporon. Histological examination revealed that the administration of L-AMB induced a transformation from acute purulent inflammation caused by both azole-susceptible and -resistant strain infections to a chronic and subsiding form, whereas FLC failed to convert the acute inflammation induced by the azole-resistant strain to a subsiding form. Our results demonstrate that polyene macrolides can be used as an alternative therapy for infection of azole-resistant strains of Trichosporon and that histopathological evaluation is useful for elucidating the pathophysiology of an experimental Trichosporon infection.

Corresponding author

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