Direct Modification of mRNA by Ferrocenyl Carbodiimide and Its Application to Electrochemical Detection of mRNA

Kosuke MUKUMOTO; Takahiko NOJIMA; Shinobu SATO; Michinori WAKI; Shigeori TAKENAKA

doi:10.2116/analsci.23.115

Original Papers

Direct Modification of mRNA by Ferrocenyl Carbodiimide and Its Application to Electrochemical Detection of mRNA

Kosuke MUKUMOTO, Takahiko NOJIMA, Shinobu SATO, Michinori WAKI, Shigeori TAKENAKA

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JOURNAL FREE ACCESS

2007 Volume 23 Issue 1 Pages 115-119

DOI https://doi.org/10.2116/analsci.23.115

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Abstract

Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probe-immobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level.

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