The growth of four fungal species (four isolates) from bathrooms was examined under various environmental conditions. These fungi were found several times in bathrooms and washing machines, but not in other indoor environments such as house dust or windows. The four species (Ramichloridium strelitziae, Cyphellophora laciniata, Phoma fimeti, and Exophiala sp.) were identified using DNA and morphological analyses. These bathroom fungi were able to consume surfactants, soap and shampoo, but were unable to grow well in high-temperature or dry conditions. Soap and shampoo seem to affect fungal flora in the bathroom.