Flagellar class 3 operons of Escherichia coli and Salmonella are transcribed by RNA polymerase containing σ²⁸. The consensus sequence of the σ²⁸-dependent pro-moters was believed to be TAAA N15 GCCGATAA. In this study, we found that the E. coli genome contains a large number of sequences homologous to this consen-sus. However, we showed that they do not always exert a σ²⁸-dependent promoter activity. We compare more carefully the sequences of the class 3 flagellar promot-ers and propose a revised structure of the σ²⁸-dependent promoters as TAAAGTTT N11 GCCGATAA.