Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
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Purification and Characterization of Corrinoid-Compounds from the Dried Powder of an Edible Cyanobacterium, Nostoc commune (Ishikurage)
Fumio WATANABEYuri TANIOKAEmi MIYAMOTOTomoyuki FUJITAHiroyuki TAKENAKAYoshihisa NAKANO
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2007 Volume 53 Issue 2 Pages 183-186

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Abstract

Vitamin B12 content (98.8±5.6 μg/100 g dry weight) of an edible cyanobacterium, Nostoc commune (Ishikurage) was determined by the Lactobacillus delbrueckii ATCC 7830 microbiological method. Bioautography with vitamin B12-dependent Escherichia coli 215 indicated that N. commune contained two (main and minor) corrinoid-compounds. These corrinoid-compounds were purified to homogeneity from the dried algal cells and characterized. The main and minor purified corrinoid-compounds were identified as pseudovitamin B12 and vitamin B12, respectively, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-visible spectroscopy. These results suggest that the bacterial cells are not suitable for use as a vitamin B12 source, especially in vegetarians.

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© 2007 by the Center for Academic Publications Japan
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