The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
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Protective Effects of Interleukin-10 on Diaphragm Muscle in a Septic Animal Model
Azusa TanedaChiyohiko ShindohYuzuru OhuchiKunio Shirato
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1998 Volume 185 Issue 1 Pages 45-54

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Abstract

The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline+endotoxin group as control and an IL-10+endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline+endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p<0.001 and p<0.05, respectively) compared to those at 0 hour. In the IL-10+endotoxin group, the decrement in the force-frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline+endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-10+endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.

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© 1998 Tohoku University Medical Press
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