Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains

Tatsuya HIGASHI; Tadashi NISHIO; Hiroyuki YOKOI; Yasuaki NINOMIYA; Kazutake SHIMADA

doi:10.2116/analsci.23.1015

Notes

Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains

Tatsuya HIGASHI, Tadashi NISHIO, Hiroyuki YOKOI, Yasuaki NINOMIYA, Kazutake SHIMADA

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JOURNAL FREE ACCESS

2007 Volume 23 Issue 8 Pages 1015-1019

DOI https://doi.org/10.2116/analsci.23.1015

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Abstract

A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5α-androstane-3α,17β-diol (3α,5α-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3α,5α-Adiol level by immobilization stress was also analyzed using the developed method.

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