Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes

Takehiko KAKIZAKI; Nobuyuki HAMADA; Seiichi WADA; Tomoo FUNAYAMA; Tetsuya SAKASHITA; Tsutomu HOHDATSU; Tadashi SANO; Masahiro NATSUHORI; Yasuhiko KOBAYASHI; Nobuhiko ITO

doi:10.1269/jrr.0618

Regular Paper

Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes

Takehiko KAKIZAKI, Nobuyuki HAMADA, Seiichi WADA, Tomoo FUNAYAMA, Tetsuya SAKASHITA, Tsutomu HOHDATSU, Tadashi SANO, Masahiro NATSUHORI, Yasuhiko KOBAYASHI, Nobuhiko ITO

Author information

Takehiko KAKIZAKI
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Nobuyuki HAMADA
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine
Seiichi WADA
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Tomoo FUNAYAMA
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Tetsuya SAKASHITA
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Tsutomu HOHDATSU
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences
Tadashi SANO
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences
Masahiro NATSUHORI
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences
Yasuhiko KOBAYASHI
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine
Nobuhiko ITO
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

Keywords: Feline T-lymphocytes, Apoptosis, Mitotic catastrophe, Clonogenic survival, Ionizing radiation

JOURNAL FREE ACCESS

2006 Volume 47 Issue 3+4 Pages 237-243

DOI https://doi.org/10.1269/jrr.0618

View "Advance Publication" version (September 6, 2006).

Details

Abstract

We have examined in vitro radiosensitivities and radioresponses to ⁶⁰Co γ-rays irradiation in feline T-lymphocyte cell lines, FeT-J and FL-4. There seemed to be no significant difference in clonogenic survival between the two lines. The mean lethal dose for both was both 1.9 Gy, and surviving fraction at 2 Gy was 0.30 and 0.48 for FeT-J and FL-4 cells, respectively. However, TUNEL assay indicated much higher degrees of apoptosis induction in FeT-J cells (>40%) than in FL-4 cells (<10%) at 4 days after 15 Gy irradiation. Microscopic examination revealed a larger population of multi-nucleate cells in FL-4 cells (60.3%) than in FeT-J cells (16.0%) at 4 days after 15 Gy irradiation, suggesting that a larger ratio of mitotic catastrophe occurred in FL-4 cells. These results suggest that FeT-J is more likely to be induced into apoptosis and FL-4 is more likely to fall into mitotic catastrophe, and eventually necrosis; both of them showed a similar surviving fraction against γ-rays. The results also indicate that FL-4 cells follow a process other than apoptosis to cell death, suggesting the presence of a regulatory mechanism that may control the relationship between mitotic catastrophe and apoptosis in feline T-lymphocytes.

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